Feeder-dependent and feeder-independent iPS cell derivation from human and mouse adipose stem cells.
Optimized multiparametric immunophenotyping of umbilical cord blood cells by flow cytometry. Isolation of adipose derived stem cells and their induction to a chondrogenic phenotype.
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Immobilization of growth factors on solid supports for the modulation of stem cell fate. Generation of induced pluripotent stem cells from human cord blood cells with only two factors: Oct4 and Sox2. Isolation and cultivation of human keratinocytes from skin or plucked hair for the generation of induced pluripotent stem cells. A protocol for isolation and culture of mesenchymal stem cells from mouse bone marrow. Isolation of a slowly adhering cell fraction containing stem cells from murine skeletal muscle by the preplate technique. Derivation and maintenance of human embryonic stem cells from poor-quality in vitro fertilization embryos.
Culture of embryonic-like stem cells from human umbilical cord blood and onward differentiation to neural cells in vitro. Author s : Karlijn J. Wilschut, Esther P. Tjin, Henk P. Monolayer culturing and cloning of human pluripotent stem cells on laminin based matrices under xeno-free and chemically defined conditions.
Somatic Stem Cells
Microinjection of membrane-impermeable molecules into single neural stem cells in brain tissue. Adapting human pluripotent stem cells to high-throughput and high-content screening. Arrayed cellular microenvironments for identifying culture and differentiation conditions for stem, primary, and rare cell populations. Studying cell behavior in whole zebrafish embryos by confocal live imaging including an application to hematopoietic stem cell emergence. An adherent cell culture of the mouse subependymal zone to study the behavior of adult neural stem cells at a single cell level.
Embryonic and induced pluripotent stem cell staining and sorting using live cell fluorescence imaging probe CDy1. Fabrication of synthetic polymer coatings and their use in feeder-free culture of human embryonic stem cells. Dynamic suspension culture for scalable expansion of undifferentiated human pluripotent stem cells.
Assaying stem cell mechanobiology on microfabricated elastomeric substrates with geometrically modulated rigidity. In vivo imaging of transplanted hematopoietic stem and progenitor cells in mouse calvarium bone marrow. Quantitation of human mammary epithelial stem cells with in vivo regenerative properties using a subrenal capsule xenotransplantation assay. A protocol describing the genetic correction of somatic human cells and subsequent generation of iPS cells. An efficient and easy-to-use cryopreservation protocol for human ES and iPS cells.
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In vivo evaluation of human hematopoiesis through xenotransplantation of purified hematopoietic stem cells from umbilical cord blood. Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification.
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Optimized mouse ES cell culture system by suspension growth in a fully defined medium. A protocol describing the use of a recombinant protein-based, animal product-free medium APEL for human embryonic stem cell differentiation as spin embryoid bodies. Production of chimeras by aggregation of embryonic stem cells with diploid or tetraploid mouse embryos.
Using a neonatal rat system as a bioincubator to generate adult-like mature cardiomyocytes from human and mouse pluripotent stem cells. This protocol was used in a related article published in Cell Reports. Generation of human high-purity ventral midbrain dopaminergic progenitors for in vitro maturation and intracerebral transplantation.
This protocol was used in a related article published in Cell Stem Cell. Directed differentiation and long-term maintenance of epicardial cells from human pluripotent stem cells under fully defined conditions. This protocol was used in a related article published in Nature Biomedical Engineering. This protocol was used in a related article published in Nature and is an Extension of an earlier Nature Protocols article. Constructing cellular niche properties by localized presentation of Wnt proteins on synthetic surfaces.
This protocol was used in a related article published in Stem Cell Reports.
Differentiation of cardiomyocytes and generation of human Engineered Heart Tissue. Generation of multipotent induced cardiac progenitor cells from mouse fibroblasts and potency testing in ex vivo mouse embryos. Pratik A. Lalit, Adriana M. Rodriguez, Karen M. Directed differentiation of human induced pluripotent stem cells into functional cholangiocyte-like cells. Co-ordinated generation of multiple ocular-like cell lineages and fabrication of functional corneal epithelial cell sheets from human iPS cells.
Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells. Reprogramming of mouse retinal neurons and standardized quantification of their differentiation in 3D retinal cultures. Daniel J. Hiler, Marie E. Barabas, Lyra M. Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.
Nesmith, Kevin K. Cardiac differentiation of human pluripotent stem cells in scalable suspension culture. Generation and isolation of oligodendrocyte progenitor cells from human pluripotent stem cells. Reprogramming fibroblasts toward cardiomyocytes, neural stem cells, and hepatocytes by cell activation and signaling directed lineage conversion. Efficient generation of functional CFTR-expressing airway epithelial cells from human pluripotent stem cells. The in vitro generation of lung and airway progenitor cells from human pluripotent stem cells. Nature Biotechnology. The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor-like cells.
Generation, expansion and functional analysis of endothelial cells and pericytes derived from human pluripotent stem cells. Directed differentiation of embryonic origin-specific vascular smooth muscle subtypes from human pluripotent stem cells. Generation of a vascularized and functional human liver from an iPSC-derived organ bud transplant. In vitro sperm production from murine spermatogonial stem cell lines using an organ culture method.
Directed differentiation of forebrain GABA interneurons from human pluripotent stem cells. Generation of eggs from mouse embryonic stem cells and induced pluripotent stem cells. Derivation of extraembryonic endoderm stem XEN cells from mouse embryos and embryonic stem cells. Laura Menendez, Michael J. Kulik, Austin T. Page, Sarah S. Park, James D. Lauderdale, Michael L. Cunningham and Stephen Dalton. Natl, Acad. Efficient introduction of specific p53 mutations into murine embryonic fibroblasts and embryonic stem cells.
Simultaneous Isolation of Three Different Stem Cell Populations from Murine Skin
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